1.the main formulation of the preparation material: (1) cottonseed shell 89%, rice bran or bran 10%, gypsum 1%; (2) sawdust 80%, rice bran or bran 20%; (3) corncob 73%, drum 25%, gypsum 1%, and sucrose 1%. Mix the above culture materials with water, then adjust the pH value to about 6. The water content is that the water is dripping out of the hand kneading material, but no water is formed. After the mixture is ready, the plastic bag with a specification of (x long) 17cmx28cm and a thick 0.6mm will be selected, loading 300g or so. Sawdust of sawdust is good for broad-leaved tree sawdust. The sawdust of cedar fir is not suitable for cultivation. The sawdust is mainly moisturizing and ventilating. It has many sawdust, easy to dry culture, fine wood chips and poor permeability. Rice bran and wheat bran contain all nutrients needed for growth and development of Flammulina velutipes. However, rice bran or bran are slow to produce bacteria, and easy to produce mushrooms, with low yield and poor quality. When the medium is bagged, it should be loaded into the same medium, so that the bacteria are identical, while the mushrooms are ready to be inoculated under sterile conditions, and the bacteria can be attached to the two ends of the bag. This should be carried out in sterile room or near sterile condition. The strain should choose suitable strains with strong vitality, strong and no bacteria contamination.
2. After inoculation, the cultivation bag was put into a clean culture room to cultivate bacteria, and the indoor temperature remained 22-25. Under normal conditions, the mycelium could be extended into the medium 20-25mm after 10-15 days of inoculation. If the bacteria are smooth, the mycelium can cover the whole material 20-25 days after the inoculation. At this stage, it is required to pick out the miscellaneous bacteria, and the bacteria bags with no full sacks are arranged together to continue to hair bacteria. After scratching bacteria and buds, it is necessary to scratch the bacteria immediately after the end of the fungus. The following bacteria can be dug away from the culture foundation surface. If the medium surface is dry, it can be scratched, that is, the old fungus skin and the 5-10mm medium are scraped off together. The mycelium can form the primordial base by mechanical stimulation and low temperature stimulation. To keep the air humidity 90%-95% and strengthen the ventilation, it can inhibit the formation of the aerogenic mycelium formed on the surface of the bag, which is beneficial to the mushroom. In this period, the relative humidity of air should be kept about 95%, and the temperature control at 10-15 C is good. It can cover or cover the film with a newspaper or cover the moisture to induce the mushroom. After a week, the tip like buds grow in succession, and the white of the bud is normal.
3. the average fertility and suppression are the low temperature of the inhibition process, so that the weak resistant primordium will not die, increase resistance and develop evenly. The temperature requirement is about 8 degrees, and the air humidity is 85%-90%, usually 2-3 days. The inhibition is to control the growth of the elongated Flammulina velutipes, and promote the elongation of the long Flammulina velutipes, so that all the length of the stalk is in accordance with the length of the fungus, and the length of the stalk is 1mm, the diameter of the cap is 1.5mm, the temperature of the inhibition period is kept at 3-5, and the humidity is 85%-90%. The concentration of CO2 is below 0.1%, and the inhibition period is about 7 days. It can also use cooling, dehumidification and ventilation measures to slow down the formation of fruiting bodies and synchronize them so as to make mushrooms neat and batch
4. Management of the growth stage, when the stalk length is 0.5-1.0cm, will increase light and increase yield and quality. As the fruiting body of Flammulina velutipes has a strong direction of light, it begins to set the paper tube to the harvest when the mushroom body is long 2-3cm. A certain light can induce the stalk to elongate to the light. Therefore, a 15W bulb is suspended every 3-5M above the bed frame to produce vertical light and promote the elongation of the stalk. At this stage, the temperature is 8-15 C, the air relative humidity is 85%-90%, the content of CO2 in the mushroom room is 0.10%-0.15%, which can achieve the purpose of inhibiting the cap opening and promoting the elongation of the stalk. About 15 days, the diameter of the cap can reach 1-2cm, and the stalk length can reach 8-15cm.
5, the suitable period was collected when the stalk was long 13-14cm, neatly, the diameter of the cap was about 1cm, the edge was not distorted, the lid of the stalk was not absorbed, the root root of the stalk was clear, round and coarse, all pure white, the mushroom body was strong, but the water content was only the harvest time. The moisture content of the cap should be checked a few days before harvest.
If there is much moisture, ventilation should be carried out 2 days before harvest. After harvesting, the part of the culture medium, which is connected with the base of the fungus and the medium of the culture medium, and the bad growing mushroom are removed. The small packaging is carried out according to the market requirement, or the polyethylene film bag is used to seal the gas, and the storage is preserved at low temperature. Two after the tide mushroom, in order to increase the output and quality, the bacteria can be removed from the head and bagged, and the original mushroom pocket can also be closed.
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